E.CLONI BAC-OPTIMIZED 25RXN

BAC-Optimised Electrocompetent Cells

E. cloni 10G BAC-Optimized Electrocompetent Cells Competent Cells include Control DNA and Recovery Medium, and are packaged as FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Product information

We offer two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimised Replicator™ v2.0 Electrocompetent Cells (> 1 × 10¹⁰ cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of CopyRight^® Cloning Kits.

E. cloni 10G BAC-Optimised Electrocompetent Cells (> 1 × 10⁷ cfu/µg 150 kb BAC DNA; > 1 × 10¹⁰ cfu/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning

SDS 60215 E. cloni 10G BAC Optimized Electrocompetent Cells

Manuals

E cloni 10 G BAC Optimized Electro

BAC Optimized Replicator

BAC Optimized Replicator v2.0 Electrocompetent Cells