Description
Eliminate RNase activity.
Supplied at 40,000 U/mL in 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol, pH 7.5 @ 25°C.
RNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.
Product specifications and usage
Unit Definition: One unit is defined as the amount of enzyme required to inhibit by 50% the hydrolysis of cytidine 2',3'-cyclic monophosphate by 5 ng of RNAse A.
Source: A recombinant E. coli strain carrying the porcine RNase Inhibitor gene.
| Unit Concentration | 40,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | 2000 U <5.0% released |
| DS Exonuclease | 2000 U <0.5% released |
| Endonuclease | 2000 U <10% converted |
| Non-specific RNAse | 2000 U none detected |
| E. coli 16S rDNA Contamination | 2000 U <10 copies |
| Storage | -20 °C |