Description
Outstanding cDNA synthesis.
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25 °C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25 °C. 250,000 U is supplied as 5 × 50,000 U.
Product information
M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.
Product specifications and usage
Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37 °C using poly r(A)/oligo (dT) as a substrate.
Source: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.
| Unit Concentration | 200,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | 2,000 U <5.0% released |
| DS Exonuclease | 2,000 U <0.5% released |
| Endonuclease | 2,000 U <10% converted |
| E. coli 16S rDNA Contamination | 2,000 U <10 copies |
| Storage | -20 °C |
SDS
MANUAL
PIS