The QuantSeq-Flex Targeted RNA-Seq Modules complement the QuantSeq FWD kits and enable the generation of targeted RNA-Seq libraries from any RNA sample using custom primers for first or second strand synthesis. A QuantSeq FWD core kit has to be purchased in addition.
If dual-targeted RNA-Seq (custom primers in first and second strand synthesis) is required, please contact to request an optimized product.
With the QuantSeq-Flex First Strand Synthesis Module V2 the reverse transcription primer of QuantSeq FWD can be substituted with custom target-specific primers. Using the QuantSeq-Flex Second Strand Synthesis Module V2 custom primers can be used for the second strand synthesis. (Please note that the second strand synthesis reaction requires annealing temperatures of >45 °C. )
For multiplexing of up to 384 samples/lane, we recommend to combine the QuantSeq-Flex Modules with QuantSeq FWD with UDIs (Cat. No. 113 – 115, 129 – 131).
The new QuantSeq-Flex First Strand Synthesis Module V2 (Cat. No. 166) can further be used to modulate the insert size or to enable larger RNA volumes for standard QuantSeq FWD library preps. For details, please consult the User Guide for the QuantSeq-Flex First Strand Synthesis Module.
- Gene expression studies of custom targets
- Targeted sequencing
- Molecular barcoding (UMI or inline indexing)
- First Strand Synthesis Module (Cat. No. 166.96)
- or Second Strand Synthesis Module (Cat. No. 028.96)
- HiSeq 2000/2500/3000/4000
- NextSeq 500/550/2000
- NovaSeq 6000
- Genome Analyzer