RNAssure Elution Tubes replace the manufacturer’s supplied elution collection tubes and integrates seamlessly with standard column-based purification kits and protocols.
| STORAGE CONDITION | LENGTH OF PROTECTION |
|---|---|
| Room temperature (15-25 °C) | 3 days |
| Refrigerator (2-8 °C) | 2 weeks |
| Frozen (more than -70 °C) | Less than 1 year |
The amount of RNase contamination varies widely depending on sample types and extraction methods, and it is extremely difficult to assess the level of contamination in an extracted RNA sample. RNAssure increases RNA stability in the liquid state at 37 °C even in the presence of trace amounts of RNase.
HeLa cell RNA (5 μg) was incubated with the indicated amounts of RNase at 37 °C for one hour in the presence (top row) or absence (bottom row) of GenTegra RNAssure.
Keeping RNA samples on ice slows degradation. However, during normal experimental handling, the sample temperature can often rise above 0 °C. Even brief exposure to elevated temperature is detrimental to RNA, especially with prevalent contamination from endogenous or environmental RNases1,2.
Within less than 15 minutes of exposure at RT, unprotected RNA shows noticeable degradation with progressive deterioration of the RNA quality over longer exposure times.
RNAssure protected RNA shows no significant change in RNA quality throughout the 4-hour exposure to RT.
Following short incubations at RT, commercially sourced purified mouse spleen RNA samples were analyzed on a Bioanalyzer™ for both fragmentation length and RIN scores. In the absence of RNAssure (orange), the RNA sample started to show noticeable degradation, after as short as 15 min exposure at RT, and the RNA quality progressively decreased as the exposure time increased. Whereas in the presence of RNAssure (green), the RNA sample was protected with no significant change in RINe value, throughout the 4 h exposure to RT. Unprotected control samples (orange), GenTegra RNAssure protected samples (green).
In the presence of RNAssure, no significant degradation was observed compared to control RNA for a minimum of 3 days and up to 7 days at RT.
Following 3 or 7 days of incubation at RT, commercially sourced purified mouse spleen RNA samples were analyzed on a Bioanalyzer for both fragmentation length and RINe scores. A. Prior to incubation at RT, control mouse spleen RNA showed no significant degradation. B. After 7 days at 25 °C, RNA showed severe degradation in the absence of RNAssure. C. Whereas, in the presence of RNAssure, no significant degradation was observed compared to control RNA. D. At 25 °C, it is observed that RIN values for the unprotected RNA (orange) showed a dramatic decrease at 3 days, while the RNAssure protected RNA (green) maintained high RINe scores even after 7 days.
The presence of RNAssure provides added protection against damage to the RNA during the DNase treatment at elevated temperature.
RNAssure protects RNA during DNase Treatment. RNA was extracted from various mouse organs using either Trizol-based method or a leading commercial column-based kit A. While mouse liver RNA extracted by Trizol was free of gDNA contamination, column-based kit led to significant contamination of gDNA of various sizes (red arrows). B. RNA extracted from brain, intestine, lung, and kidney, using column-based method, was subjected to in-solution DNase treatment following manufacturer’s instruction (37°C for 30 min). After treatment, RNA from different samples showed moderate to severe degradation, evidenced by decreased 18S rRNA (top band) vs 28S rRNA (bottom band). In contrast, RNAssure helped significantly to maintain the RNA integrity during DNase digestion.
