Description
As the proven alternative to BigDye ® Terminator Cycle Sequencing Kits, the SupreDye™ Cycle Sequencing Kits provide all the sequencing-required components in a ready, pre-mixed format to deliver the high-quality performance you expect with no changes necessary protocol, system settings, or calibrations, at a superior value. Both single-stranded or double-stranded DNA templates from PCR and plasmids as well as large templates and G-rich templates (GT and GC) can be sequenced with uniform peak heights and long, high-resolution reads.
Versions 1.1. and 3.1 of the SupreDye Cycle Sequencing Kits are alternatives for BigDye Terminator Cycle Sequencing v1.1 (for shorter reading or reading near primer) and v3.1 (for longer reading such as de novo sequencing and resequencing purpose), respectively. The SupreDye dGTP v1.0 and 3.0 Cycle Sequencing Kits are designed for sequencing G-rich templates with greater success and they are alternatives for BigDye ® dGTP Terminator Cycle Sequencing v1.0 and v3.0, respectively.
- Proven high-quality performance
- Uniform peak heights and long, high-resolution reads
- Increased robustness for difficult to sequence templates
- Direct, Drop-in Substitute!
- No change necessary in protocol, system settings, or calibrations.
- Fully compatible with all ABI genetic analyzers
Kit Components:
- SupreDye reaction ready sequencing premix
- pGEM control DNA
- M13 control primer
- 5x sequencing buffer
SDS
SupreDye v3.1 Cycle Sequencing Kit.pdf
SupreDye Enhancer.pdf