Description
The SNAPDye v1.1 Terminator Cycle Sequencing Kit is based on the Sanger Dideoxynucleotide Chain Termination method. The kit is provided with a 2.5x concentrated ready-reaction premix, plus sequencing dilution buffer and controls (plasmid DNA template and sequencing primer) fully optimized for a highly flexible chemistry, designed for all kinds of sequencing applications, including de novo sequencing and resequencing. The v1.1 kit generates data with uniform peak heights and optimized signal balance to produce long, high-quality reads that require optimal base calling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules. In addition, the v1.1 kit may provide more accurate base assignments for heterozygote and mutation detection.
Components:
Protocol:
The SNAPDye Terminator Kit was developed as a direct, drop-in substitute for BigDye® Terminator v1.1, with no changes in protocol, dilution, workflow, calibration or settings using DyeSet E. Simply dilute the SNAPDye Cycle Sequencing Mix and substitute it for BigDye® Terminator as described in your SOP. Using 0.5 - 1.0 μl of SNAPDye Cycle Sequencing Mix (1:16 - 1:8 dilution) produces results that are indistinguishable from BigDye® Terminator v1.1 in comparison reactions with uniform peak heights, extremely low background and long sequence reads with high confidence scores.