AmpFi RT MasterMix (DM - AmpRT ) ensures accurate qPCR results where contaminating gDNA would otherwise result in artificially - early Ct values. RNA samples contaminated with gDNA were reverse - transcribed for 25 min with AmpFi RT MasterMix . 1μI of RT product was then used in qPCR to assay amplification of a GAPDH target.
Store at -20°C.
AmpFi RT MasterMix was designed to perform simple, reliable, and reproducible first-strand cDNA synthesis.
• AmpFi Reverse Transcriptase
• Ribonuclease Inhibitor
• Temperature-sensitive DNase,
• Oligo (dT)s and Random Primers.
|AmpFi RT MasterMix||100 rxn (400 μl)|
|Nuclease-Free H2O||1.0 ml|