Store at -20°C.
AmpFi HS DNA Polymerase is a Taq Polymerase that has rapid extension rates and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a reaction set-up at room temperature without non-specific amplification and primer dimer formation. This polymerase provides increased processivity, yields, and sensitivity, and reduced reaction times by up to 70%, compared to wild-type Taq DNA polymerase when conditions are optimized.
The antibody dissociates from the DNA polymerase during the initial denaturation step, restoring enzyme activity. Hence, reducing non-specific amplification and competition for reagent availability. This specificity increase improves the yield of PCR products. AmpFi HS DNA Polymerase has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with the polymerase can be used with TA cloning vectors.
|AmpFi HS DNA Polymerase||400 rxn (200 μl)|
|AmpFi HS Buffer1||2 x 1.0 ml|
*Buffer contains 1.5 mM Mg2+