AmpFi HS DNA Polymerase

DMarkSKU: DM-AmpHS400

Size: 400 rxns



Store at -20°C.

AmpFi HS DNA Polymerase has rapid extension rates and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a reaction set-up at room temperature without non-specific amplification and primer dimer formation. This polymerase provides increased processivity, yields, and sensitivity, and reduced reaction times by up to 70%, compared to wild-type Taq DNA polymerase when conditions are optimized.
The antibody dissociates from the DNA polymerase during the initial denaturation step, restoring enzyme activity. Hence, reducing non-specific amplification and competition for reagent availability. This specificity increase improves the yield of PCR products. AmpFi HS DNA Polymerase has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with the polymerase can be used with TA cloning vectors.

Product Component Quantity
AmpFi HS DNA Polymerase 400 rxn (200 μl)
AmpFi HS Buffer1  2 x 1.0 ml

 *Buffer contains 1.5 mM Mg2+



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