Description
plexWell™ 384 (PW384) Highlights:
- plexWell NGS multiplexed library generation kit for Illumina®
- Assay-ready 96-well fully-skirted low-profile PCR plates (4 x 96)
- 2,304 (96 x 24) unique barcode combinations
- 6 sets of 4 pool barcodes – PB Set A, B, C, D, E, or F
Description:
- Kit contains major reagents necessary to prepare libraries including Magwise™ paramagnetic beads (DNA polymerase not included)
- Efficient library prep for more than one 96-well plate of samples
- Normalizes input DNA over wide input range of 3-30 ng
- Save 30-50% on total lab costs
Recommended Applications:
- Large scale full length viral surveillance
- Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc)
- Microbiome screening
- Microbial whole genome sequencing
- scRNA-seq
- Low-depth whole genome/GBS
plexWell™ Library Prep Workflow:
plexWell™ Library Prep Chemistry:
Specifications:
-
Time-to-prepare a multiplexed library from 96 samples:
- Hands-on time: 55 min
- Total elapsed time: 3 hrs
- Recommended input: 3-30 ng of gDNA per sample (n=96)
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Expected Results:
- Library Yield: 750 – 1500 fmoles of purified multiplexed library
- Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
- Substantial normalization: Typically <2.5-fold range (min to max) in read count across all 96 samples
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Compatibility with Illumina sequencing systems:
- Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms
Downloads:
plexWell Kits Master Index List
plexWell DNA Input Global Dilution Calculator
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