Description
Parallel isolation of RNA and protein from an undivided sample
Specifications:
| Application | Isolation of RNA and protein |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Handling | Centrifugation |
| Lysate clarification | Centrifugation, NucleoSpin Filter |
| Automated use | No |
| Sample material | Animal tissue, Cells, Human tissue, Plant |
| Sample amount | < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue |
| Fragment size | RNA > 200 nt, protein 15–300 kDa |
| Typical yield | < 70 µg RNA, < 1200 µg Protein |
| Theoretical binding capacity | 200 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Typical RIN | > 9 |
| Elution volume | RNA 40–120 µL, protein 10–100 µL |
| Preparation time | 30 min/6 preps (RNA), + 35 min/6 preps (protein) |
| Typical downstream application | Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |