Description
Optimised, complete system for production of full-length cDNA from low amounts of total RNA.
Kit contains MMLV Reverse Transcriptase, 10X Reaction Buffer, RiboGuard RNase Inhibitor, dNTP PreMix, DTT, Oligo(dT)21 Primer, Random 9-mer Primers, RNase-free Water.
Key features
- Synthesise full-length cDNA (15 kb)
- Generate first-strand cDNA from picogram amounts of total RNA
- Select oligo(dT) or random primers - both included
Product information
The MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit produces full-length first-strand cDNA from total cellular RNA preparations or purified poly(A) RNA.
This complete kit includes the same MMLV High Performance Reverse Transcriptase that is available as a separate component (Item ID. RT80125K) and maintains all the same high performance characteristics as that stand alone enzyme. To help simplify your cDNA synthesis, this kit contains all the necessary components to make 1st strand cDNA.
Applications
- First-strand cDNA synthesis for subsequent PCR or real-time PCR.
- RT-PCR validation of gene expression data obtained from microarray experiments.
- RT-PCR validation and quantification of gene silencing by RNA interference.
Benefits
- Synthesise full-length cDNA from RNA templates longer than 15 kb.
- Reaction Buffer, optimised for producing full-length cDNA, is included with both the MMLV HP RT and the MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit.
- The kit includes both an oligo(dT) and random nonamer primers.
- First-strand cDNA can be made from picogram amounts of input total RNA.
- The kit includes a potent RNase Inhibitor to protect the integrity of template RNA.
