Description
Ampligase Thermostable DNA Ligase starter Kit (Item ID A8101) includes Ampligase Thermostable DNA Ligase, 10X Ampligase Reaction Buffer, and a DNA ligation control.
All other Ampligase Thermostable DNA Ligase Kits include Ampligase DNA Ligase and 10X Ampligase Reaction Buffer.
Ampligase™ Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1 hour at 95°C. Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C/80°C) or 16 hours of cycling. This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. Ampligase DNA Ligase has no detectable activity in ligating blunt-ended DNA and has no activity on RNA or RNA:DNA hybrids.
Key Features:
High thermostability allows ligation using high-stringency hybridization conditions
High specificity and stringency permits sensitive detection of SNPs
Available in kit and "enzyme only" formats
Applications
- Ligation amplification (Ligase Chain Reaction, LCR)
- Repeat Expansion Detection (RED)
- Simultaneous mutagenesis of multiple sites
- Other ligation-based detection methods
Documentation:
A0110K A3210K Ampligase DNA Ligase
A0102K A32750 A3202K Ampligase Enzyme and Buffer