truCOVER WGS PCR Free - 96

Sample Type: DNA from blood, saliva, FFPE, cell culture, and microbes, among other sources

Application: Whole Genome Sequencing with or without Amplification, Compatible with Whole Exome Sequencing and Targeted Gene Panel workflows

Covaris truCOVER^™ DNA Library Prep Kits deliver unmatched flexibility and performance. Prepare high-quality libraries from as little as 0.1 ng or as much as 500 ng of DNA across a wide range of input amounts. Whether working with blood, saliva, buccal swabs, FFPE, cell culture, or microbial DNA, truCOVER ensures reliable and reproducible creation of NGS libraries with a single, streamlined workflow.

Tunable mechanical fragmentation with proven Adaptive Focused Acoustics^® (AFA^®) technology enhances our Library Kit workflow to provide tight control over insert sizes, regardless of GC content, while optimized reagents simplify each step.

• High-quality library yield, with negligible duplication rate and insert size bias. Yields can be boosted up to 6x through 4x amplification without compromising fragment integrity.
• Consistent and uniform library sizes via easily tunable fragmentation, even for low-input or degraded samples 
• Operational efficiency (e.g., 30% reduced turnaround time) via simplified workflow. High-quality library generation in just 2 hours and 50 minutes for input samples ≥50 ng. Libraries from low-input samples needing amplification can be constructed in 3 hours and 50 minutes with 25 minutes of hands-on time.
• Sequence-independent uniform and unbiased libraries and batch-to-batch consistency enables optimized flow cell utilization
• Wide sample input range (0.1 - 500 ng) across sample types (blood, saliva, FFPE, cell culture and microbial DNA) in one workflow with or without amplification, adding workflow flexibility
• Specially formulated clean-up reagents included in the kit, which is designed to reduce downstream sequencing instrument fragment size bias, enabling simplified library pooling by mass or equal volume without additional steps

truCOVER Workflow with Optional Amplification

Kit Contents

 The truCOVER WGS PCR-free Library Prep Kit (520361) contains enough reagents to process 96 samples of 50 – 500 ng without the need for amplification. 

The truCOVER Library Amplification Kit (520366) is an add-on kit for processing low-input samples from 0.1 – 50 ng. Order the truCOVER DNA Library Prep Kit with Amplification (520361 and 520366) for the complete library preparation of low-input samples.  

Specifications