NucleoSpin RNA
NucleoSpin RNA
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NucleoSpin RNA
NucleoSpin RNA

NucleoSpin RNA

Macherey-NagelSKU: MN-740955.250
Size: 250
Price:
$2,136

Description

Mini spin kit for isolation of RNA of highest integrity

Specifications:

Application Isolation of RNA
Target RNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Package unit 10 Prep(s), 50 Prep(s), 250 Prep(s), QIAcube 240 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification Centrifugation, NucleoSpin Filter
Automated use No
Sample material Animal tissue, Bacteria, Cells, Human tissue, Yeast
Sample amount < 5 x 10? cultured cells, < 30 mg human / animal tissue, < 10? bacterial cells, < 10? yeast cells
Fragment size > 200 nt
Typical yield 14 µg (10? HeLa cells), 70 µg (10? bacterial cells)
Theoretical binding capacity 200 µg
Typical purity A260/A280 1.9–2.1
Typical RIN > 9
Elution volume 30–120 µL
Preparation time 35 min/6 preps
Typical downstream application RNA-Seq, RT?PCR
Storage temperature 15?25 °C
Shelf life (from production) 24 Months
Hazardous material Yes

Isolation of RNA of highest quality and integrity

The NucleoSpin RNA kit from MACHEREY?NAGEL offers tailored and specialized solutions that will allow you to purify high quality RNA from a variety of sample materials.

In contrast to the RNeasy kit from QIAGEN, our NucleoSpin RNA kit includes rDNase and NucleoSpin filters. So there is no need for additional orders, as customers receive everything needed for a successful, DNA-free RNA purification with the MN kit. And it minimizes the risk of getting stuck mid-process due to lack of consumables.

The purified RNA allows downstream analysis in a variety of applications including enzymatic labeling reactions (e. g. dye incorporation), reverse transcriptase-PCR (RT-PCR) and RNA-sequencing.

Product workflow overview – 35 min/6 preps

Comparative data MN vs. QIAGEN

Matching quality, optimum value, reliable service

Our comparative study of NucleoSpin RNA and QIAGEN RNeasy demonstrates equivalent performance across key metrics (upper two panels, liver sample). Both kits yield high-quality RNA with comparable RIN values (9.2 vs. 9.6) and total yields (20.1 vs. 18.7 ?g). Results of qRT-PCR show similar CT values for various genes across different sample types, indicating consistent RNA purity and integrity (lower two panels). For researchers seeking a costeffective alternative without compromising quality, NucleoSpin RNA offers equivalent performance to the market leader, with the added benefit of included rDNase and personalized support from our product specialists in the field. If you’re a current QIAGEN user, consider trying NucleoSpin RNA for your next RNA purification – you’ll experience comparable results with optimum convenience and customized service.


Appreciated by scientists worldwide

Our NucleoSpin RNA kits are highly appreciated by scientists due to their high quality, fast and easy workflows and our comprehensive service. Only in the last two years, the NucleoSpin RNA kit has been cited in several hundred peer-reviewed publications. The following reference list shows some of the latest publications from 2023 and 2024 on NucleoSpin RNA listed by title and publication year of the study, journal and impact factor, as well as the downstream application.


Downloads:

Manual

Support Protocols:

User Guide - RNA purification guide

Application Note - RNA isolation from bacteria and yeast

Tech Note - RNA isolation from insects

Reference list: 

NucleoSpin RNA, Mini kit for RNA isolation: 740955.10/.50/.250

Title Journal IF  Downstream application
Preclinical models for prediction of immunotherapy outcomes and immune evasion mechanisms in genetically heterogeneous multiple myeloma. Nature Medicine 87,2   qRT-PCR
A druggable copper-signalling pathway that drives inflammation. Nature 64,8 RNA-seq
Resistance of HNSCC cell models to pan-FGFR inhibition depends on the EMT phenotype associating with clinical outcome. Molecular Cancer 41,4 RNA-seq
The therapeutically actionable long non-coding RNA 'T-RECS' is essential to cancer cells' survival in NRAS/MAPK-driven melanoma. Molecular Cancer 41,4 qRT-PCR
Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway. Nature Immunology 31,2 Microarray, qRT-PCR
Optimization of the proliferation and persistency of CAR T cells derived from human induced pluripotent stem cells. Nature Biomedical Engineering 29,3 Whole transcriptome sequencing
Colorectal Cancer Organoid-Stroma Biobank Allows Subtype-Specific Assessment of Individualized Therapy Responses. Cancer Discovery 28,2 RNA-seq
Therapeutic activity of GARP:TGF-?1 blockade in murine primary myelofibrosis. Blood 20,3 RNA-seq
Polymorphism in interferon alpha/beta receptor contributes to glucocorticoid response and outcome of ARDS and COVID-19. Critical Care (London, England) 19,3 RNA-seq
Modulation of Dendritic Cell Function via Nanoparticle-Induced Cytosolic Calcium Changes. Acs Nano 17,1 RNA-seq
Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr. Nature Communications 16,6 qRT-PCR, RNA pulldown assay, RNA immuniprecipitation
Reversible transitions between noradrenergic and mesenchymal tumor identities define cell plasticity in neuroblastoma. Nature Communications 16,6 RNA-seq
Leukemia relapse via genetic immune escape after allogeneic hematopoietic cell transplantation. Nature Communications 16,6 RNA-seq
Diversity of the Pacific Ocean coral reef microbiome. Nature Communications 16,6 qRT-PCR
Mechanistic characterization of a Drosophila model of paraneoplastic nephrotic syndrome. Nature Communications 16,6 qRT-PCR
T-cell stimulating vaccines empower CD3 bispecific antibody therapy in solid tumors. Nature Communications 16,6  
RHBDL4-triggered downregulation of COPII adaptor protein TMED7 suppresses TLR4-mediated inflammatory signaling. Nature Communications 16,6 qRT-PCR
Plasticity-induced repression of Irf6 underlies acquired resistance to cancer immunotherapy in pancreatic ductal adenocarcinoma. Nature Communications 16,6 qRT-PCR
A ubiquitin-based effector-to-inhibitor switch coordinates early brain, craniofacial, and skin development. Nature Communications 16,6 qRT-PCR
SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation. Nature Communications 16,6 qRT-PCR
PAX4 loss of function increases diabetes risk by altering human pancreatic endocrine cell development. Nature Communications 16,6 qRT-PCR
N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer's disease mouse model. Nature Communications 16,6 RNA-seq
Cellular reprogramming in vivo initiated by SOX4 pioneer factor activity. Nature Communications 16,6 qRT-PCR
Mtfp1 ablation enhances mitochondrial respiration and protects against hepatic steatosis. Nature Communications 16,6 RNA-seq, qRT-PCR
Non-invasive assessment of normal and impaired iron homeostasis in the brain. Nature Communications 16,6 RNA-seq
Intratumoral androgen biosynthesis associated with 3?-hydroxysteroid dehydrogenase 1 promotes resistance to radiotherapy in prostate cancer. The Journal of Clinical Investigation 15,9 RNA-seq
SF3B1 mutation and ATM deletion codrive leukemogenesis via centromeric R-loop dysregulation. The Journal of Clinical Investigation 15,9 RNA-seq
Neutrophil CEACAM1 determines susceptibility to NETosis by regulating the S1PR2/S1PR3 axis in liver transplantation. The Journal of Clinical Investigation 15,9 qRT-PCR
Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner. The Journal of Experimental Medicine 15,3 qRT-PCR
PPAR? Acetylation Orchestrates Adipose Plasticity and Metabolic Rhythms. Advanced Science (Weinheim, Baden-Wurttemberg, Germany) 15,1  
Highly Mimetic Ex Vivo Lung-Cancer Spheroid-Based Physiological Model for Clinical Precision Therapeutics. Advanced Science (Weinheim, Baden-Wurttemberg, Germany) 15,1  
LncRNA ARGI Contributes to Virus-Induced Pancreatic ? Cell Inflammation Through Transcriptional Activation of IFN-Stimulated Genes. Advanced Science (Weinheim, Baden-Wurttemberg, Germany) 15,1  
Surveillance of 3' mRNA cleavage during transcription termination requires CF IB/Hrp1. Nucleic Acids Research 14,9 RNA-seq
Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants. Nucleic Acids Research 14,9 qRT-PCR
Enhancer-promoter interactions can form independently of genomic distance and be functional across TAD boundaries.

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