Description
NucleoSpin Plasmid Transfection-grade Plus is the new generation for silica membrane-based plasmid isolation. This kit allows the isolation of up to 100 µg of ultrapure plasmid DNA with endotoxin levels ≤ 1 EU/µg plasmid DNA.
| Application | Transfection-grade plasmid DNA isolation |
| Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology and endotoxin reduction technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Handling | Centrifugation, Vacuum |
| Lysate clarification | Centrifugation |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 1−5 mL |
| Vector size | < 25 kbp |
| Typical yield | 35-60 µg |
| Theoretical binding capacity | 100 µg |
| Typical purity A260/A280 | 1.8−1.85 |
| Endotoxin level | ≤ 1 EU/μg DNA |
| Elution volume | 30−50 µL |
| Preparation time | 20 min/6 preps |
| Typical downstream application | Transfection of sensitive cells and cell injections |
| Storage temperature | 15–25 °C / 59–77 °F |
| Shelf life (from production) | 24 Month(s) |
| Hazardous material | Yes |