Description
The UltraMarathonRT® Two-Step RT-PCR Kit is a next-generation solution for researchers needing sensitive and faithful RNA-to-cDNA conversion followed by amplification in RT-PCR experiments, regardless of RNA template length, structure, or GC content.
The kit is designed for experiments requiring high sensitivity and precision, significantly enhancing gene detection with lengths ranging from hundreds of nucleotides to tens of thousands of nucleotides. Unlike other RT-PCR kits, the UltraMarathonRT RT-PCR Kit requires only 0.1 pg of RNA input for the amplification of RNAs > 10 kb.
UltraMarathonRT is an ultra-processive reverse transcriptase enzyme with intrinsic helicase activity that actively unwinds RNA secondary and tertiary structures naturally without heat, faithfully producing full-length cDNAs from complex, long, or structured RNA templates that standard reverse transcriptases often miss. The DNA polymerase provided in this kit has high fidelity and is extremely processive in amplifying long cDNA (up to 30 kb) generated by UltraMarathonRT.
This kit is perfect for researchers looking to achieve high-quality results using RT-PCR working with long, complex RNA and includes all necessary reagents.
Key Applications:
- Gene expression analysis
- Full-length isoform analysis
- Full-length virus genome analysis
- Molecular cloning
- Long or structured RNA analysis with low input
Kit Components:
- UltraMarathonRT
- 2x RT Buffer
- High Boost
- Nuclease-free Water
- dNTP mix (10 mM each)
- 2x PCR Master Mix
- Random primers (15mer) (10 µM)
- Oligo(dT)18 Primer (5 μM)
Catalog# R1005S (20 rxns), R1005M (50 rxns), R1005L (250 rxns)
Reagents required but not supplied: RNA template: 0.1 pg-2 μg total cellular RNA or 0.1 pg-500 ng of mRNA are recommended for each reaction. If target template is > 10 kb, a minimum input of 1 pg is recommended for both total cellular RNA or mRNA.
Storage conditions: For short duration of storage (< 3 month), UltraMarathonRT can be placed in a -20℃ freezer. -80℃ is recommended for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.
A High-Performance Solution for Sensitive and Precise RNA Analysis
Convert and Amplify with PrecisionKey Features
Unparalleled Sensitivity
Requires as little as 0.1 pg of input RNA to amplify RNAs >10 kb, enabling detection of even low-abundance targets.
Sensitivity of uMRT two-step RT-PCR for RNA input as low as 0.1 pg
Processive Enzyme
Powered by UltraMarathonRT®, an ultra-processive reverse transcriptase with intrinsic helicase activity that actively unwinds RNA secondary and tertiary structures without heat, producing full-length cDNA with exceptional fidelity.
Long-Read Capability:
Efficiently amplifies cDNA products up to 30 kb using a highly processive, high-fidelity DNA polymerase.
Amplification of transcripts from 1.2 kb to 14.9 kb using 100 ng Hela total RNA. The cDNA’s generated by Induro RT were amplified by LongAmp Taq. Reverse transcription was performed using Oligo(dT) 18 primer.
Versatile Applications
Ideal for experiments requiring accurate gene detection, from short transcripts to tens of thousands of nucleotides in the areas of gene expression analysis, full-length isoform analysis, full-length virus genome analysis, molecular cloning, long or structured RNA analysis with low input.
UltraMarathonRT Two-Step RT-PCR Kit
Perfect for researchers working with long, complex RNA templates who demand high-quality RT-PCR results with maximum sensitivity and precision.
Downloads:
Random primers (15mer) (10 µM) SDS
uMRT Two-Step RT-PCR Kit Protocol