Achieve a more accurate and comprehensive view of the transcriptome—at the same cost as leading kits. Click the cards below to explore how the cDNA Synthesis & Amplification Kit, powered by uMRT, delivers deeper insights from RNA samples.
Description
The UltraMarathonRT® (uMRT) cDNA Synthesis & Amplification Kit enables you to see more of the transcriptome in each sample. uMRT is a next-generation reverse transcriptase that synthesizes end-to-end cDNA in a single pass from even the most complex RNA molecules—long, structured, high-GC, or low-abundance.
Conventional retroviral RTs are ‘distributive’ meaning they attach and detach many times from each RNA transcript which often creates truncated, incomplete, or incorrect cDNAs. This is often caused by secondary structures or repeat sequences within the template.
UltraMarathonRT solves this problem. It is a highly engineered group II intron-encoded reverse transcriptase with intrinsic helicase activity. It bypasses the drop-offs, biases, and blind spots common in traditional cDNA synthesis workflows.
Why Choose This Kit
- Higher Gene Detection & Isoform Sensitivity
Captures more genes and isoforms, including alternative splicing and intron retention events that other kits miss. - Superior Amplification Accuracy
Uniform gene body coverage improves the accuracy of differential gene expression analysis. -
Designed for Modern RNA-Seq Applications
Perfect for short-read sequencing, biomarker discovery, and therapeutic target validation.
Applications
- Bulk RNA-Seq
- Short-read RNA-Seq
- Low-abundance transcript detection
- Isoform-level resolution
- Alternative splicing analysis
- RNA biomarker discovery
UltraMarathonRT cDNA Synthesis & Amplification Kit Components:
- UltraMarathonRT (20 U/µL)
- 2x RT Buffer S
- dNTP mix (10 mM each)
- dATP solution (10 mM)
- uMRT oligo(dT) primer (5 μM)
- uMRT-TSO_v2
- AmpPCR
- 5x Template Switching Buffer
- Nuclease-free water
- 5x DNA Polymerase Buffer
- DNA Polymerase (1 U/µL)
Reagents Required but Not Supplied:
- RNA template: 1 ng – 100 ng total cellular RNA or 0.1 ng – 20 ng mRNA is recommended for each reaction.
- Post PCR clean-up reagent.
Product Information
Catalog# R1007S (20 reactions), R1007M (50 reactions), R1007L (100 reactions)
Storage Conditions: For short duration of storage (< 6 month), UltraMarathonRT can be stored in a -20℃ freezer. -80℃ is recommended for long-term storage. All other kit components are stable in a -20℃ freezer for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.
Uncover the RNAs that retroviral reverse transcriptases miss.Switch to the UltraMarathonRT-powered cDNA Synthesis & Amplification Kit
RNA-seq is a powerful method for studying gene expression, but commonly used retroviral reverse transcriptases (e.g., MMLV and AMV) lack two critical properties for effective reverse transcription: intrinsic helicase activity, which enables the unwinding of complex RNA secondary structures, and high processivity, which allows for the synthesis of full-length cDNA from long RNA molecules.
UltraMarathonRT (uMRT) is a reverse transcriptase enzyme designed to overcome these limitations and improve RNA coverage. The cDNA Synthesis & Amplification Kit, powered by uMRT, combines intrinsic helicase activity with ultraprocessivity to efficiently reverse transcribe long RNA sequences (>30 kb—compared to the ~2 kb typically transcribed by retroviral RTs), as well as RNA with high GC content, complex structures, or low abundance. uMRT also functions at lower temperatures, helping preserve RNA integrity and enabling a more comprehensive view of the transcriptome.
Power through RNA complexity and boost sample coverage
The UltraMarathonRT cDNA Synthesis & Amplification Kit enables superior quantification of:
- Highly structured RNAs
- Long RNAs
- Transcripts with high GC content
- Non-coding RNAs
Greater visibility into the transcriptome
10-30% more genes
Better RNA Quantification
Less Bias
2x More Retained Introns
For Research Use Only.
Downloads:
uMRT oligo(dT) primer (5 μM) SDS
5x Template Switching Buffer SDS
cDNA Synthesis & Amplification Protocol