Description
PhiRx Indexed Control is an easy-to-use solution to maximize the performance of XLEAP-SBS chemistry on high-throughput Illumina platforms by color-balancing both the sequencing reads and barcodes.
- Optimizes index diversity for XLEAP sequencing
- Serves as a seamless spike-in sequencing control, similar to Illumina’s PhiX Control V3
- Corrects index color balancing issues for both low diversity barcodes and small batch sequencing runs
- Improves signal uniformity and data quality
- Helps recover data that would be lost as undetermined reads
A Color-Balanced Spike-In Control for Improved Demultiplexing on XLEAP-SBS Illumina Sequencing Instruments
What is PhiRx?
PhiRx Indexed Control is a dual-indexed control library, made from phiX174 genomic DNA, optimized for Illumina sequencing platforms, particularly two-color systems like XLEAP SBS chemistry on NextSeq 1000/2000 and NovaSeq X. By improving color balancing for low-plexity libraries PhiRx ensures cleaner, more accurate sequencing results.
Key Features
- 10 bp i5 and i7 indexes with mixed bases: Provides high base diversity, automatically meeting color balancing requirements on supported platforms. (patent-pending)
- Color balancing optimization: Specifically designed for the sensitive XLEAP SBS chemistry, enabling as little as 5% spike-in to correct base calling issues in libraries with low-diversity indexes.
- High diversity for low-plexity samples: Randomized inserts with 45% GC content improve sequencing accuracy for low-diversity libraries.
- Reliable quality control: Derived from the well-characterized phiX174 genome, providing robust calibration to support sequencing accuracy and performance.
PhiRx Indexed Control reduces the rate of data loss from 53% to < 2%
| Spike-in Sequencing Control | % PhiX Aligned | % Undetermined Reads (Excluding PhiX) | % One-mismatch |
| seqWell PhiRx Indexed Control | 5.22 | 1.82 | 4.15 |
| Illumina PhiX Control V3 | 6.27 | 53.25 | 100.0 |
A 96-plex library prepared using ExpressPlex 2.0 was sequenced on a NextSeq 2000 with 5% spike-in of Illumina PhiX Control V3 or PhiRx from seqWell.
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