Description
The truChIP® Chromatin Shearing Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from cultured mammalian cells (suspension or adherent) using the Covaris Adaptive Focused Acoustics® (AFA®) technology.
Kit contains truCHlP reagents, formaldehyde, and protocol for chromatin shearing of 10-30mm cells (High Cell; 15 samples) or 1-3mm cells (Low Cell; 50 samples). To be used with milliTUBE with AFA Fiber or microTUBE with AFA Fiber.
Specifications:
| Technical Specification | Description |
|---|---|
| Part Number | COV-520154 |
| Application | Chromatin Shearing |
| Instrument Compatibility | LE220-plus, LE220R-plus, ME220, E220, E220evo, M220, S220 |
Quick Guide:
truChIP® Chromatin Shearing Kit
SDS:
Safety Data Sheet Reverse Crosslinking Buffer
Safety Data Sheet Protease Inhibitor Cocktail
Chromatin Preparation and Shearing
Safety Data Sheet 16% Formaldehyde, Methanol Free
Safety Data Sheet Halt Protease Inhibitor Cocktail
Safety Data Sheet 10X Fixing Buffer
Safety Data Sheet 10X SDS Shearing Buffer D3
Safety Data Sheet 5X Lysis Buffer
Safety Data Sheet 2X IP Dilution Buffer 3X IP Dilution Buffer
Safety Data Sheet 1X Quenching Buffer
Posters:
Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo
Brochures:
Comprehensive Epigenomic Sample Prep Workflow Solutions
truChIP® Chromatin Sample Preparation Solutions