PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.
The PippinHT instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
| PippinHT Specs | |
| Electrophoresis voltage | 75V constant, 75V pulsed field |
| Optical detection | 470 nm excitation, 525 nm emission |
| Power requirements | 100-240 VAC, 2.5 A, 50-60 Hz |
| Weight | 20 lbs / 9 kg |
| Dimensions (with lid open) | 18h x 13w x 17d (in) | 46h x 33w x 43 d (cm) |
Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.
Citations:
Paired-End Sequencing
Sheaffer, K.L. et al., DNA methylation is required for the control of stem cell differentiation in the small intestine. Genes Dev. 28: 652-664 (2014)
Mate-Pair Sequencing
Srivastava, A. et al., Discovery of transgene insertion sites by high throughput sequencing of mate pair libraries. BMC Genomics. 15:367 (2014)
Long-Read Sequencing
Benson, M.A. et al., Evolution of hypervirulence by a MRSA clone through acquisition of a transposable element. Molecular Microbiology 93(4), 664-681 (2014)
miRNA Isolation
Singhal, R. et al. FOXO1 regulates expression of a microRNA cluster on X chromosome. AGING, 5:5. (2013)
ChIP-seq
Wharton, K., et al., Methylation and Next-Generation Sequencing of free circulating DNA from human plasma. BMC Genomics, 15:476 (2014
