Description
Extract up to 1MB DNA directly from cells
The HLS2 system provides a new approach to DNA extraction. Users load cells suspensions or cell nuclei on Sage Science gel cassettes to perform lysis under electrophoretic conditions. This removes degraded and solubilized proteins but leaves intact DNA behind, bound in agarose. A cleavase is then used to carefully fragment the DNA into electrophoretically-mobile sizes. Automated DNA size selection collects the DNA in 6 size bins. Learn more about the technology.
Targeted Purification with CRISPR/Cas9: CATCH*
CRISPR/Cas9 can be used to design targeted purification of large genomic regions or genes. This in vitro application with Cas9, termed CATCH, can enhance study of structural variation and provide valuable phasing information.
*Cas9-Assisted Targeting of CHromosome Segments
Jiang et. al., 2015, Nature Communications, doi:10.1038/ncomm9101
Key Downstream Technologies
- optical mapping
- long read sequencing
- bar-coded linked reads
Instrument
The SageHLS instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
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| SageHLS Specifications | |
| Electrophoresis voltage | 25V - 100V pulsed field and 150V constant |
| Optical detection | None |
| Power requirements | 100-240 VAC, 2.5 A, 50-60 Hz |
| Weight | 20 lbs / 9 kg |
| Dimensions | 10.5h x 13w x 10d (in) | 27h x 33w x 25d (cm), w/ closed lid |
Cassette Kits
Kits include 4 or 10 ea. disposable pre-cast gel cassettes. Cassette accommodate 2 samples. Some reagents must purchased separately.