Description
Automation of NGS library preparation on the Revvity Sciclone® G3 NGSx workstation reduces sample tracking errors and sample-to-sample variability while dramatically increasing throughput. Samples are processed in 96-well PCR plates, and the number of samples to be processed (1 to 12 columns of 8 samples each) is selected at the start of each run. Pre-set tip-tracking utilities written into the Maestro applications guide the instrument to pick up appropriate numbers of tips and refill/replace tip boxes as needed. Inheco temperature blocks installed on the Sciclone® deck allow for appropriate 4°C storage of reagents and controlled incubation temperatures for enzymatic steps. Reaction mixes are pre-arrayed prior to sample addition to eliminate incubation time variability between samples. The easy-to-follow user interfaces guide the reagent and deck setup process and prompt the user for any necessary interventions.
The NEXTFLEX® Rapid Directional RNA-Seq Automation kit 2.0 is designed to prepare directional, strand-specific RNA libraries from total RNA if used in conjunction with the NEXTFLEX® Poly(A) Beads 2.0 (10 ng - 5 µg) or NEXTFLEX® RiboNaut™ rRNA depletion kit (human, mouse, rat) (5 ng - 1µg) for sequencing using Illumina® sequencers. The workflow is broken into three separate applications and can be completed in approximately 9-10 hours.