Description
Method of choice for His-tag protein purification with best performance in 96‑well plate format
Specifications:
| Application | Purification of His-tag proteins |
| Target | Protein |
| CE certified | No, research use only |
| Technology | Immobilized metal ion affinity chromatography |
| Chelating ligand | NTA (nitrilotriacetic acid) |
| Brand | Protino |
| Format | 96-well plate |
| Package unit | 96 Prep(s), 384 Prep(s) |
| Handling | Vacuum, centrifugation |
| Automated use | Yes |
| Application note available with | Hamilton |
| Sample material | Protein solution |
| Matrix | 6 % beaded agarose (cross-linked), precharged with Ni²⁺ |
| Bead size | 45–165 µm |
| Bed volume | Variable (50 µL recommended) |
| Theoretical binding capacity | 3 mg/well (50 µL of settled agarose) |
| Storage temperature | 4−8 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |