Description
Flexible magnetic bead based isolation of DNA and RNA from water and air samples
Specifications:
| Application | Isolation of RNA and DNA |
| Target | DNA |
| CE certified | No, research use only |
| Technology | Magnetic bead technology |
| Brand | NucleoMag |
| Format | Magnetic beads |
| Package unit | 96 Prep(s), 384 Prep(s) |
| Handling | Magnetic separation |
| Automated use | Yes |
| Sample material | Air, Water |
| Sample amount | 10–1000 mL |
| Fragment size | 300 bp–approx. 50 kbp |
| Typical yield | Depending on amount and quality of sample |
| Theoretical binding capacity | 0.4 µg/µL beads |
| Elution volume | 50–250 µL |
| Preparation time | 40–120 min/96 preps (excl. lysis) |
| Typical downstream application | Next Generation Sequencing, qPCR, RT-PCR |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |
Support Protocols:
viral nucleic acid isolation from water samples
sample concentration for viral nucleic acids from water
Technical Notes:
Viral nucleic acid isolation from wastewater samples
INNOVAPREP wastewater concentration
SARS-CoV-2 extraction from wastewater concentrated with Nanotrap Magnetic Virus Particles
Learn More
NucleoMag DNA/RNA water
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Isolation of RNA and DNA The NucleoMag DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality though determination of the microbial load of the sample. |
Automated and manual purification of DNA and RNA from filters
NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
Compatibility with different filtration systems
NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex). 
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.
Various water samples can be processed with NucleoMag DNA/RNA Water.
DNA and RNA can be extracted from a range of water samples, spanning from turbid to clear, as well as from air filters. 
Efficient detection for different water, and air samples
Various water samples, and an air sample were filtered, and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag DNA / RNA Water kit.
Research Use Only