Description
XS spin kit for ultrafast and convenient RNA isolation without rDNase digestion
Specifications:
| Application | Isolation of RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | XS prep |
| Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Handling | Centrifugation |
| Lysate clarification | Centrifugation, NucleoSpin gDNA Removal Column |
| Automated use | No |
| Sample material | Animal tissue, Cells, Human tissue |
| Sample amount | 1−10⁵ cultured cells, < 5 mg human / animal tissue |
| Fragment size | > 100 nt |
| Typical yield | 0.05−0.2 ng (10¹ HeLa cells ), 0.5−2 µg (10⁵ HeLa cells), 2.5−8 ng (0.5 µg mouse liver), 0.1−0.5 ng (0.5 µg mouse brain) |
| Theoretical binding capacity | 110 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Typical purity A260/A230 | 1.5−2.5 |
| Typical RIN | > 9 |
| Elution volume | 5–30 µL |
| Preparation time | 18 min/6 preps |
| Typical downstream application | Array technology, Northern blotting, Primer extension, Real−time RT−PCR |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |