Description
Mini spin kit for isolation of small and large RNA from formalin-fixed, paraffin-embedded samples
Specifications:
| Application | Isolation of total RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Handling | Centrifugation, vacuum |
| Lysate clarification | Centrifugation |
| Automated use | No |
| Sample material | FFPE, Fixed samples, Tissue sections |
| Sample amount | 10 sections (10 μm) with < 50 mg of tissue |
| Fragment size | > 18 nt |
| Typical yield | Depending on amount and quality of sample |
| Theoretical binding capacity | 200 µg |
| Elution volume | 30–50 µL |
| Preparation time | 70 min/6 preps (90 min incl. optional rDNase digest) |
| Typical downstream application | Array technology, Northern blotting, Primer extension, qRT−PCR, RNase protection assays |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |