Description
High troughput purification of small and large constructs
Specifications:
| Application | High and low copy plasmid DNA purification |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Filtration |
| Brand | NucleoSpin |
| Format | 96-well plate |
| Package unit | 192 Prep(s), 384 Prep(s), 2304 Prep(s) |
| Handling | Centrifugation, vacuum |
| Lysate clarification | NucleoSpin Flash Filter Plate |
| Automated use | Yes |
| Sample material | Bacteria, E. coli |
| Sample amount | 1.1−1.3 mL (high copy), 1.1−3.9 mL (low copy) |
| Vector size | < 250 kbp |
| Typical yield | 8 µg (high copy), 1 µg (low copy) |
| Typical purity A260/A280 | 1.8−1.85 |
| Endotoxin level | > 50 EU/µg DNA |
| Preparation time | 90 min/2 plates |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 27 Months |
| Hazardous material | Yes |