Description
Rapid Mini preparation of plasmid DNA
Specifications:
| Application | High and low copy plasmid DNA purification |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Handling | Centrifugation, vacuum |
| Lysate clarification | Centrifugation |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 1−5 mL (high copy), 6−10 mL (low copy) |
| Vector size | < 25 kbp |
| Typical yield | 25−45 µg |
| Theoretical binding capacity | 60 µg |
| Typical purity A260/A280 | 1.8−1.85 |
| Endotoxin level | > 50 EU/µg DNA |
| Elution volume | 50 µL |
| Preparation time | 20 min/6 preps |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 27 Months |
| Hazardous material | Yes |
Support Protocols: