Description
1st generation anion exchange technology for large construct plasmid DNA
Specifications:
| Application | Large contruct (P1, BACs, PACs) plasmid purification |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | BAC prep |
| Package unit | 10 Prep(s) |
| Handling | Gravity flow |
| Lysate clarification | Folded filter |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 100−500 mL E. coli culture |
| Vector size | < 300 kbp |
| Typical yield | 10−100 µg |
| Theoretical binding capacity | 100 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | 1−10 EU/µg DNA |
| Isopropanol precipitation | Centrifugation |
| Preparation time | 120 min/2−4 preps |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Scope of delivery | NucleoBond BAC 100 Columns, NucleoBond Folded Filters, buffers, RNase A |
| Hazardous material | Yes |