Description
1st generation anion exchanger for purification of plasmid DNA
Specifications:
| Application | High and low copy plasmid DNA purification |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Mega prep |
| Package unit | 5 Prep(s) |
| Handling | Gravity flow |
| Lysate clarification | Folded filter |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 150−500 mL (high copy); 500−2000 mL (low copy) |
| Vector size | < 300 kbp |
| Typical yield | 500−2000 µg |
| Theoretical binding capacity | 2000 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | 1−10 EU/µg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi |
| Preparation time | 90−120 min/4−6 preps |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Scope of delivery | NucleoBond AX 2000 Columns, NucleoBond Folded Filters, buffers, RNase A |
| Hazardous material | Yes |