Description
NucleoSpin 96 Plasmid Transfection-grade Plus is the new generation for silica membrane-based plasmid isolation in a 96-well plate. This kit allows the isolation of up to 60 µg of ultrapure plasmid DNA with endotoxin levels ≤ 1 EU/µg plasmid DNA.
| Application | High copy plasmid DNA purification |
|---|---|
| Automated use | Yes |
| Automation support | Compatible with most open robotic platforms. Please visit www.mn-net.com/automation or consult our technical support for further information. |
| Brand | NucleoSpin |
| CE certified | No, research use only |
| Elution volume | 75−150 µL |
| Endotoxin level | ≤ 10 EU/μg with 3 wash steps , ≤ 1 EU/μg with 4 wash steps |
| Format | 96-well plate |
| Handling | Centrifugation, Vacuum |
| Hazardous material | Yes |
| Lysate clarification | NucleoSpin Plasmid Filter Plate |
| Preparation time | 50 min/plate |
| Sample amount | 1−5 mL |
| Sample material | Bacteria, E. coli |
| Selling unit | 96 Prep(s), 384 Prep(s) |
| Shelf life (from production) | 24 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Plasmid DNA |
| Technology | Silica membrane technology and endotoxin reduction technology |
| Theoretical binding capacity | 60 µg |
| Typical downstream application | Transfection of sensitive cells and cell injections |
| Typical purity A260/A280 | 1.8−1.85 |
| Typical yield | 15-50 µg |
| Vector size | < 25 kbp |
Downloads:
Instruction NucleoSpin 96 Plasmid TF-Grade Plus
Leaflet - NucleoSpin Plasmid Transfection-grade Plus
Flyer NucleoSpin Plasmid TG Plus EN
Application Note - NucleoSpin 96 Plasmid TG Plus - epMotion 5075vt Eppendorf
Application Note NucleoSpin 96 Plasmid Transfection-grade Plus on Andrew+ Waters