Description
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA
Specifications:
| Application | Endotoxin-free plasmid DNA isolation |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | 96-well plate |
| Package unit | 96 Prep(s), 384 Prep(s) |
| Handling | Gravity flow , vacuum for filtration and precipitation |
| Lysate clarification | NucleoBond Filter Plate |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 1−5 mL |
| Vector size | < 25 kbp; < 300 kbp (without the NucleoBond Finalizer Plate) |
| Typical yield | 2−4 µg (1.5 mL in 96-well plates), 10−50 µg (5 mL in glass tubes) |
| Theoretical binding capacity | 50 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | < 0.1 EU/µg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer Plate |
| Elution volume | 100−200 µL |
| Preparation time | 120 min/plate |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Hazardous material | Yes |