Description
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA
Specifications:
| Application | Endotoxin-free plasmid DNA isolation |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Package unit | 10 Prep(s), 50 Prep(s) |
| Handling | Gravity flow |
| Lysate clarification | Column filter |
| Automated use | Yes, can be automated on the Andrew+ the pipetting robot |
| Sample material | Bacteria, E. coli |
| Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
| Vector size | < 300 kbp |
| Typical yield | 500 µg |
| Theoretical binding capacity | 800 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | < 0.1 EU/µg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
| Preparation time | 85 min/prep |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Hazardous material | Yes |
Outstanding yields and ultra-pure plasmid DNA
Use NucleoBond Xtra Midi/Maxi EF to get the most out of your plasmid DNA prep!
Direct comparison of total plasmid yields (pcDNA3.1) grown in LB medium isolated with NucleoBond Xtra Midi EF and Maxi EF as well as different competitor kits according to the respective user manual.
Endotoxins in EU/μg DNA determined after plasmid DNA isolation with NucleoBond Xtra Midi EF and Maxi EF show levels below the FDA limit for vaccines (<0.04 EU/μg DNA).
The direct comparison data show that anion exchange technology has obvious advantages over silica membrane technology in terms of yields and quality. It demonstrates that this technology is still gold standard for purification of high quality plasmid DNA. MN offers with the NucleoBond Xtra Midi/Maxi EF product series excellent kits delivering the quality required for your downstream application in an extremely short processing time.