Description
Total RNA extraction from all kinds of sample
Specifications:
| Application | Isolation of total RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | One phase extraction |
| Brand | NucleoZOL |
| Format | Reagent |
| Package unit | 200 mL |
| Handling | Centrifugation |
| Lysate clarification | Centrifugation |
| Automated use | No |
| Sample material | Animal tissue, Bacteria, Cells, Human tissue, Plant, Viral fluid, Yeast |
| Sample amount | < 2 x 10⁶ cultured cells, < 2 x 10⁶ bacterial cells, < 2 x 10⁶ yeast cells, < 100 mg human /animal / plant tissue, < 0.4 mL viral fluid |
| Fragment size | Total RNA > 10 nt (small RNA 10-200 nt, large RNA > 200 nt) |
| Typical yield | 1–8 µg total RNA/mg sample |
| Typical purity A260/A280 | 1.8–2.1 |
| Typical RIN | > 9 |
| Elution volume | Flexible |
| Preparation time | < 1 h |
| Typical downstream application | Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Scope of delivery | NucleoSpin RNA Columns, Collection Tubes, buffers |
| Hazardous material | Yes |