Description
Optimised reverse transcriptase and buffer system for the production of full-length cDNA.
Kit contains MMLV High Performance Reverse Transcriptase, 10X Reaction Buffer, and DTT.
Key features
- Synthesise full-length cDNA (15 kb)
- Amplify first-strand cDNA from picogram amounts of total RNA
- Optimise the RT reaction to your specific needs by using the first strand cDNA synthesis primers, dNTPs and RNase inhibitor of your choice (not included).
Product information
The MMLV HP RT demonstrates significant reverse transcriptase activity, typically requiring just 100 units of enzyme. MMLV HP RT includes a 10X Reaction Buffer, optimised for synthesis of full-length cDNA from long RNA templates, and DTT. The enzyme, buffer and DTT are the same components used in the MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit. By providing these three components individually, you have the flexibility to choose dNTPs, RNase Inhibitors, etc. and optimise the RT reaction for your specific needs.
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