Description
RNA is precious. Protect it with this high quality, effective RNase inhibitor.
Key features
- Inhibit RNase A, RNase B and RNase C
- Prevent RNA degradation and protect your precious samples
Product information
RiboGuard RNase Inhibitor is the best defence against common RNases, including RNase A, RNase B, and RNase C. This recombinant RNase inhibitor protein provides reliable protection of valuable RNA samples by binding strongly to RNases in a 1:1 ratio. Advanced purification protocols and extensive quality control tests ensure RiboGuard RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.
Applications
- cDNA synthesis
- RT-PCR
- in vitro transcription and translation
Benefits
- A potent affinity for RNases (Ki >10-14 M) ensures rapid inhibition even when trace amounts of RNase are present.
- Free of detectable RNase or DNase activity, and mammalian DNA.
- Does not interfere with enzymes commonly used to prepare or analyse RNA.
- Less sensitive to oxidation than traditional RNase inhibitors.
Product specifications and usage
Unit Definition: One unit of RiboGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity is measured by the inhibition of hydrolysis of 2,3´-cyclic monophosphate by RNase A.
Storage Buffer: 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 100 mM NaCl, and 10 mM DTT.
Quality Control: RiboGuard RNase Inhibitor is free of detectable DNA exo- and endonuclease, and RNase activities.
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