Description
Highly efficient, magnetic bead based purification of PCR products.
Product information
sbeadex™ PCR clean-up kits are used to purify PCR products. Components of the PCR reaction such that primers, unincorporated nucleotides, enzymes and other contaminations are efficiently removed during the purification process due to the chemical specificity and physical size of sbeadex particles. The method was developed and optimised using 20 μL of PCR product from standard PCR mixtures which were subsequently analysed by Sanger sequencing. The protocol can be scaled up or down for other volumes of PCR product, however care should be taken to maintain the buffer volume ratios specified in the protocol.