Description
Reliable and robust enzyme for production of mRNA transcripts in a variety of applications.
Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C.
Key features
- Reliable transcription.
Product information
T7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.
Product specifications and usage
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37 °C.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
| Unit Concentration | 50,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | 500 U <0.1% released |
| DS Exonuclease | 500 U <0.1% released |
| Endonuclease | 500 U <10% converted |
| RNase Contamination | 500 U = none detected |
| E. coli 16S rDNA Contamination | 500 U <10 copies |
| Storage | -20 °C |