AmpFi 2x qPCR MasterMix provides analysis of DNA samples for quantitative real-time in a convenient, reliable setup .
This qPCR MasterMix contains Taq Polymerase, DNA Polymerase, providing for rapid extension rates. This polymerase provides increased yields, and sensitivity, while optimizing reaction times by up to 70%, compared to wild-type Taq DNA polymerase.
AmpFi 2x qPCR MasterMix has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with AmpFi 2x qPCR MasterMix can be used with TA cloning vectors. The MasterMix contains dye comparable to SYBR Green™ and EvaGreen™.