M220 Focused-ultrasonicator

CovarisSKU: COV-500295



  • Treatment Power: 75W PIP
  • Sample Processing Capacity: Single tube processing
  • NGS Sample Volumes: 15 to 130 µl

The M220 Focused-ultrasonicator offers the “Scientist’s Standard” in a compact, easy-to-use system making it the ideal DNA shearing solution for MiSeq and PGM users. AFA® technology in the M220 eliminates operator-induced variations, improves recoveries, increases efficiency, and provides standardized results.



Specifications :

Technical Specification Description
Primary Applications Cell lysis: mammalian, bacterial, yeast, cfDNA Extraction from Plasma, DNA Extraction from Dried Blood Spots, DNA/RNA/Chromatin Shearing, DNA/RNA/Total NA/Protein Extraction from FFPE, Tissue lysis and extraction
Treatment Power 75W PIP
Part Number COV-500295
Power Requirements 100-240 VAC 500 VA, 50-60Hz
Operating Environment Ambient temperature: 19 °C to 25 °C (66 °F to 77 °F) Relative humidity: 30% to 70%
Operating Temperature Internal temperature control: Protocol temperature range from 7 °C to 20 °C
Safety Complies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements"
Regulatory Labeling CE, ETL Mark (for Product Safety), WEEE
SonoLab Version SonoLab 7
Operating System Windows 10
Chiller Solid state chiller built-in
Sample Processing Capacity Single tube processing
NGS Sample Volumes 15 to 130 µl
Recommended Batch Size NA
Covaris Consumables microTUBE, microTUBE-50, milliTUBE 1 ml, miniTUBE
Upgrade Potential No
Associated Accessories M220 Accessories



Key Features:

  • Controlled energy delivery
  • Isothermal processing
  • Non-contact, closed vessel
  • Quiet operation
  • Robust and reliable design
  • Tried and tested in major genome centers worldwide



  • User-selectable fragment lengths
  • No heat damage, high sample recovery
  • No cross contamination, no aerosols, no clean up
  • No discomfort to operators
  • Reproducible results, year after year
  • The mechanical shearing method of choice


Quick Guide:

DNA Shearing Protocols for Ion Torrent Platforms with the M220 Focused-ultrasonicator

Quick Guide: DNA Shearing with M220 Focused-ultrasonicator

TruSight Tumor 170 DNA Shearing Quick Guide



M220 Focused-ultrasonicator Setup & Instruction Manual



Two New Solutions for Nucleic Acid Fragmentation

Five-Minute Ultrasonic Inactivation and Protein Extraction of Mycobacteria for Identification by MALDI-ToF MS

Protein Extraction of Non-Candida Yeast using an advanced acoustic technology for MALDI-TOF Identification

Evaluation of a New Protein Extraction Method Using an Advanced Acoustic Technology for Identification of Filamentous Fungi by MALDI-TOF MS

Four Minute Mycobacterium sp. Sample Preparation Process for MALDI-TOF Identification



Formulation and Drug Delivery Breakthrough processing for poorly soluble API’s

M-Series Focused-ultrasonicators


Application Notes:

AFA-based Efficient DNA Extraction and Optional Mechanical Shearing from Mycobacteria smegmatis

DNA Extraction from mycobacteria smegmatis.  Learn More

AFA-based MALDI Biotyper Sample Preparation for Mycobacteria Colonies

AFA-based MALDI    Learn More

Analysis of DNA fragments using the Agilent 2100 Bioanalyzer

DNA shearing protocols for Focused-ultrasonicators with the Agilent DNA 12000 kit was used to evaluate the average base pair size because of the large fragment...  Learn More

DNA Shearing for Next Generation Sequencing (NGS) with the M220 Focused-ultrasonicator

DNA Shearing for NGS with the ME220 Focused-ultrasonicator.  Learn More

Magnetic Bead-based Clean-up Using AFA-energetics®

When precisely tuned, this stream of Covaris Adaptive Focused Acoustic energy enables DNA shearing precisely and reliably to desired fragment sizes, in addition to gentle...  Learn More

Protein Extraction from Yeast: Comparison of the Covaris Adaptive Focused Acoustics®(AFA) Process to Conventional Bead Beating and Probe Sonication

The efficiency of several mechanical-based lysis and extraction techniques, such as Adaptive Focused Acoustics (AFA), probe sonication, and bead beating from yeast isolates was compared....  Learn More

Simplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications

Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results....  Learn More

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