Description
AmpFi RT MasterMix (DM - AmpRT ) ensures accurate qPCR results where contaminating gDNA would otherwise result in artificially - early Ct values. RNA samples contaminated with gDNA were reverse - transcribed for 25 min with AmpFi RT MasterMix . 1μI of RT product was then used in qPCR to assay amplification of a GAPDH target.
Store at -20°C.
AmpFi RT MasterMix was designed to perform simple, reliable, and reproducible first-strand cDNA synthesis.
Reagent components:
• AmpFi Reverse Transcriptase
• Ribonuclease Inhibitor
• Temperature-sensitive DNase,
• dNTPs,
• Oligo (dT)s and Random Primers.
| Product Component | Quantity |
| AmpFi RT MasterMix | 100 rxn (400 μl) |
| Nuclease-Free H2O | 1.0 ml |
