Description
Mini spin kit for isolation of RNA of highest integrity
Specifications:
| Application | Isolation of RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s), QIAcube 240 Prep(s) |
| Handling | Centrifugation, vacuum |
| Lysate clarification | Centrifugation, NucleoSpin Filter |
| Automated use | No |
| Sample material | Animal tissue, Bacteria, Cells, Human tissue, Yeast |
| Sample amount | < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 10⁹ bacterial cells, < 10⁸ yeast cells |
| Fragment size | > 200 nt |
| Typical yield | 14 µg (10⁶ HeLa cells), 70 µg (10⁹ bacterial cells) |
| Theoretical binding capacity | 200 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Typical RIN | > 9 |
| Elution volume | 30–120 µL |
| Preparation time | 35 min/6 preps |
| Typical downstream application | RNA-Seq, RT−PCR |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |
Sheet
Support Protocols:
- Download Support protocol - Difficult to lyse tissue
- Download Support protocol - Fibrous tissue
- Download Support protocol - Disruption of RNAlater stabilized tissue using the Mixer Mill MM300
- Download Support protocol - Saliva collected with Oragene RNA
- Download Support protocol - RNA from Paxgene tubes
User Guide - RNA purification guide