NucleoMag HMW DNA, high molecular weight DNA from diverse sample materials

Macherey-NagelSKU: MN-744160.1

Price:
$559

Description

NucleoMag HMW DNA can be used for the isolation of high molecular weight DNA from cells, human and animal tissue, plant material, whole blood, saliva, swabs, as well as bacteria and yeast samples.The magnetic bead technology streamlines your workflow and enhances reliability of HMW DNA isolation due to its compatibility with diverse automation platforms.

Typical purity A260/A230 ≥ 2.0, depending on sample type and quality
Typical recovery Depending on sample amount and quality
Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Magnetic bead technology
Brand NucleoMag
Format Magnetic beads
Handling Magnetic separation
Lysate clarification Centrifugation
Automated use Yes
Automation support Compatible with most open robotic platforms. Please visit www.mn-net.com/automation or consult our technical support for further information.
Sample material Animal tissue, Bacteria, Blood, Cells, Human tissue, Plant, Saliva, Swabs, Yeast
Sample amount ≤ 20 mg human/animal tissue, ≤ 10⁶ cultured cells, ≤ 50 mg plant leaves, ≤ 25 mg gram-negative bacteria, ≤ 35 mg gram-positive bacteria, ≤ 50 mg yeast
Fragment size ≥ 50 kb - Up to 100-200 kb
Typical purity A260/A280 ≥ 1.8 , depending on sample type and quality
Elution volume 100–200 µL
Preparation time 60-120 min / 48 preps depending on manual or automated processing
Typical downstream application Long-read sequencing, Next Generation Sequencing
Shelf life (from production) 24 Month(s)
Gross weight (incl. packaging) 807.6 g / 1.78 lbs
Packaging dimensions 150 x 150 x 117 mm / 5.91 x 5.91 x 4.61 Inch
Hazardous material Yes


 Safety Data Sheet: 744160.1_en-EN_GHS.pdf

 

NucleoMag HMW DNA

High Molecular Weight DNA for Long-Read Sequencing

Automatable magnetic bead technology!

  • Reproducible isolation of HMW DNA (Up to 200 kb)
  • High yields and purity from a wide range of sample materials
  • Manual and automated processing options
HWM DNA from a wide range of sample types

High molecular weight DNA from diverse sample materials

Genomic DNA from different samples such as heart tissue, plant leaves, blood, cells, saliva, bacteria and yeast was isolated using the NucleoMag HMW DNA Kit from MACHEREY-NAGEL. Isolated DNA shows fragment lengths above 50 kb. Samples were analyzed on Agilent’s Bioanalyzer 2100. Results obtained from different fragment length analyses.

High yields and purities

HMW DNA was extracted from different sample types with the NucleoMag HMW DNA and with Supplier Z kit. MACHEREY-NAGEL´s magnetic bead based kit showed higher yields as well as purities for the tested sample types. Isolated DNA was analyzed by NanoDropTM 2000.

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