Description
The kit is a magnetic bead-based kit for the manual or automated extraction of viral RNA/DNA and bacterial DNA from veterinary sample specimens such as serum, plasma, cell-free biological fluids, animal whole blood, tissue, swabs, milk and feces. After sample treatment, samples can be processed with the standard purification protocol.
- Inhibitor free viral RNA/DNA and bacterial DNA extraction
- Highest flexibility for various veterinary sample types
- Same standard protocol for all specimen matrices after sample pretreatment
- Easy to automate on magnetic rod or liquid handler systems - reach out to us at orders@dmarbio.com for assistance
Reliable detection of BTV, CSFV, PCV 2, and SBV after DNA or RNA isolation using the NucleoMag VET kit
The NucleoMag VET kit was used to extract viral nucleic acids from bovine blood (A), porcine blood (B), porcine tissue (c) or bovine serum (d). Subsequently, qPCR-analysis was performed to determine the viral titer load in the sample materials: (A) BTV (Bluetongue virus); (B) CSFV (Classical Swine Fever virus) (C) PCV 2 (Porcine Circovirus Type 2); (D) SBV (Schmallenberg virus). Extraction performance was compared to other commercial nucleic acid extractions kits available for isolation of viral RNA and/or DNA. The qRT-PCR data show that DNA or RNA concentration varies on the extraction kit used, which results in a dissimilar detection of BTV, CSFV, PCV 2, and SBV. For the detection of the various viruses in the disparate sample materials, it is shown that DNA or RNA concentration was always high using the NucleoMag VET compared to the other kits. The obtained Ct values for the viral nucleic acids and sample materials show that the NucleoMag VET kit is a very consistent and reliable extraction kit, obtaining low Ct values for a reliable virus detection independent from virus type or sample material.
Source: Application note NucleoMag VET on KingFisher; data was kindly provided by Dr. Hoffmann, Friedrich-Loeffler-Institut, Germany.
Specifications:
| Application | Isolation of viral and bacterial nucleic acids |
| Target | Viral RNA and DNA |
| CE certified | No, research use only |
| Technology | Magnetic bead technology |
| Brand | NucleoMag |
| Format | Magnetic beads |
| Package unit | 96 Prep(s), 384 Prep(s) |
| Handling | Magnetic separation |
| Lysate clarification | Centrifugation |
| Automated use | Yes |
| Application note available with | Eppendorf, Hamilton, Thermo |
| Sample material | Animal tissue, Blood, Cell-free biological fluid, Plasma, Serum, Stool, Swabs |
| Sample amount | < 200 µL whole blood/serum/plasma/swab solution/feces, 10−30 mg tissue |
| Fragment size | 300 bp−approx. 50 kbp |
| Typical recovery | Depending on sample amount and quality |
| Theoretical binding capacity | 0.4 µg/µL beads |
| Elution volume | 50–100 µL |
| Preparation time | 40–120 min/96 preps |
| Typical downstream application | enzymatic reactions, PCR, RT-PCR |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |
Peer-reviewed publications list
Application Notes:
Processing of honey bees and pollen on Eppendorf epMotion 5075
Hamilton MICROLAB STAR and Kylt technology
Processing of blood and tissue samples on Eppendorf epMotion 5075