Description
Clone and sub-clone with high-efficiency, recA, endA competent cells.
Includes pUC19 control DNA.
Key features
- General cloning strain
- Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.
- Achieve high efficiencies with convenient chemically competent format.
- Ideal for cloning, subcloning and plasmid preparation due to endA and rec mutations.
Product information
The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table. 1).
Applications
- Routine cloning of DNA up to 200 kb.
Benefits
- High transformation efficiency with clones of all sizes, including BAC clones (Table. 1).
- lacZΔM15 for blue/white screening of recombinants.
- Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
- Endonuclease minus (endA1) to ensure high yields of DNA.
- Recombination minus (recA1) for greater stability of large cloned inserts.
| DNA | TransforMax EC100 Chemically Competent E. coli | TransforMax EC100 Electrocompetent E. coli |
| pUC19 | 1.4 × 108 | 1.4 × 1010 |
| 8.1-kb Clone | 1.3 × 107 | Not tested |
| 13.1-kb Clone | 4.3 × 106 | 1.3 × 109 |
| 23.1-kb Clone | 9.2 × 105 | 3.0 × 108 |
| 145-kb BAC Clone | Not tested | 7 × 107 |
| 13.1-kb clone directly from a ligation reaction | 2.2 × 105 | 2.1 × 107 |
Table. 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µL of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µL aliquot from a standard 10-µL ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG
TransforMax EC100 Electrocompetent E. coli
- Transformation efficiency of >1 × 1010 cfu/µg of pUC19.
TransforMax EC100 Chemically Competent E. coli
- Transformation efficiency of >5 × 108 cfu/µg of pUC19.
SDS
MANUALS