Description
High Sensitivity. High Conversion. Low-Input
The Twist cfDNA Library Preparation Kit addresses challenges associated with library preparation from circulating cell-free DNA (cfDNA). Liquid biopsies have gained prominence in oncology research, particularly for investigating potential biomarkers within genetic material from tumors circulating in peripheral blood. This kit enables high conversion cfDNA library generation for next-generation sequencing (NGS) on Illumina systems, overcoming the technical challenges associated with low-input and degradation of cfDNA, as well as other samples that are hard to acquire and have limited DNA abundance (i.e. urine and CSF samples).
The Twist cfDNA Library Preparation Kits provide our highest quality library preparation solution, offering not only robust performance with low sample input, but also high conversion rates.
The Twist cfDNA Library Preparation Kit and Twist cfDNA Library Preparation and Hyb Mix Kit enable WGS as well as targeted enrichment workflows.
- High conversion rate reliably detects rare variants (≤0.1% VAF)
- High coverage libraries compatible with duplex sequencing workflows supported by the Twist UMI Adapter System
- Robust performance, even with low (<1ng) sample input
- Sequence-ready libraries in 2 hours
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields.
Following library generation, samples were eluted in 20 μL of water and 1 µL was quantified with QubitTM dsDNA Broad Range Kit.
Twist cfDNA Library Preparation and Hyb Mix Kit enables sensitive and accurate detection of variants at 0.25% VAF.
Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
Twist cfDNA Library Preparation and Hyb Mix Kit delivers higher complexity with and without UMI deduplication.
Libraries were single-plex captured with a custom 50kb oncology panel targeting variant sites in the cfDNA standard material with present or updated TE recommendation.
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples.
Following library generation, samples were eluted in 20 μL of water and 1 μL was quantified with QubitTM dsDNA Broad Range Kit.
*For research use only. Not for use in any diagnostic or clinical procedures
- Twi-107603 - Twist cfDNA Library Preparation Kit–16 Samples
- Twi-107604 - Twist cfDNA Library Preparation Kit- 96 Samples
- Twi-107609 - Twist cfDNA Library Preparation and Hyb Mix Kit–16 Samples and 2 Reactions
- Twi- 107610 - Twist cfDNA Library Preparation and Hyb Mix Kit–96 Samples and 12 Reactions