{"product_id":"umrt-two-step-rt-pcr-kit","title":"uMRT Two-Step RT-PCR Kit","description":"\u003cp\u003e\u003cspan\u003eThe \u003cstrong\u003eUltraMarathonRT\u003csup\u003e® \u003c\/sup\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cstrong\u003eTwo-Step RT-PCR Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a next-generation solution for researchers needing sensitive and faithful RNA-to-cDNA conversion followed by amplification in RT-PCR experiments, regardless of RNA template length, structure, or GC content.\u003c\/p\u003e\n\u003cp\u003eThe kit is designed for experiments requiring high sensitivity and precision, significantly enhancing gene detection with lengths ranging from hundreds of nucleotides to tens of thousands of nucleotides. Unlike other RT-PCR kits, the UltraMarathonRT RT-PCR Kit requires only 0.1 pg of RNA input for the amplification of RNAs \u0026gt; 10 kb. \u003c\/p\u003e\n\u003cp\u003eUltraMarathonRT is an ultra-processive reverse transcriptase enzyme with intrinsic helicase activity that actively unwinds RNA secondary and tertiary structures naturally without heat, faithfully producing full-length cDNAs from complex, long, or structured RNA templates that standard reverse transcriptases often miss. The DNA polymerase provided in this kit has high fidelity and is extremely processive in amplifying long cDNA (up to 30 kb) generated by UltraMarathonRT.\u003c\/p\u003e\n\u003cp\u003eThis kit is perfect for researchers looking to achieve high-quality results using RT-PCR working with long, complex RNA and includes all necessary reagents.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Applications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression analysis\u003c\/li\u003e\n\u003cli\u003eFull-length isoform analysis\u003c\/li\u003e\n\u003cli\u003eFull-length virus genome analysis\u003c\/li\u003e\n\u003cli\u003eMolecular cloning\u003c\/li\u003e\n\u003cli\u003eLong or structured RNA analysis with low input\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eUltraMarathonRT\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e2x RT Buffer\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHigh Boost\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eNuclease-free Water\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003edNTP mix (10 mM each)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e2x PCR Master Mix\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRandom primers (15mer) (10 µM)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eOligo(dT)\u003csub\u003e18\u003c\/sub\u003e Primer (5 μM)\u003cbr\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eCatalog# R1005S (20 rxns), R1005M (50 rxns), R1005L (250 rxns) \u003c\/p\u003e","brand":"RNA Connect","offers":[{"title":"20 rxns","offer_id":45503333728300,"sku":"RNC-R1005S","price":414.0,"currency_code":"CAD","in_stock":true},{"title":"50 rxns","offer_id":45503333761068,"sku":"RNC-R1005M","price":941.0,"currency_code":"CAD","in_stock":true},{"title":"250 rxns","offer_id":45503333793836,"sku":"RNC-R1005L","price":4132.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/RNAConnect_RTPCRproductrelease_PRimage.png?v=1777656167","url":"https:\/\/dmarkbio.com\/products\/umrt-two-step-rt-pcr-kit","provider":"DMark","version":"1.0","type":"link"}