prepGEM Universal (500)

MicroGemSKU: PUN0500

Price:
$1,372

Description

MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.

  • Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
  • Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
  • Perfect for CRISPR/Cas9 genotyping workflows
  • Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer

 

Overview

prepGEM Universal is MicroGEM’s comprehensive solution for effortless high-yield DNA extraction from blood, saliva, and tissues. It is also perfectly suited for extracting DNA from cell cultures. All within a single tube, this method ensures no loss of DNA, and as a result, it excels with low cell number extractions.

This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.

prepGEM Universal utilizes our thermophilic proteinase by activation with a simple temperature change. The whole process is easily automated in a single tube using standard laboratory equipment. A standard thermocycler can be programmed to carry out the extraction.

  • Stream-lined approach provides DNA in approximately 15 minutes
  • Inhibitor-free solutions mean no harsh chemical washes or multiple steps are required
  • Closed-tube extraction protects the integrity of the sample
  • Simplified, hands-off workflow frees up technician time, reduces lab costs, and significantly reduces hazardous/plastic waste

 

MicroGEM’s single-step, closed-tube, temperature-controlled lysis and extraction is a robust solution for loss-less DNA extraction from cultured cells.

When performing gene editing via CRISPR/Cas9, genotyping with Next Gen Sequencing (NGS) is required to confirm the target gene has been successfully modified. This task requires working with very few cells. It is laborious, expensive and difficult with common purification techniques

prepGEM Universal gives linear results from 200,000 cells down to a single cell. Working volumes can be scaled to sub-microlitre volumes making it perfect for working with low cell numbers. Speed, yield and integrity make prepGEM Universal the ideal nucleic acid extraction method for researchers working with cell cultures for gene editing and expression.

Many universities, research hospitals, government entities, and private companies, have already adopted prepGEM Universal into their workflow. The single-step, closed-tube temperature-controlled lysis and extraction is highly desirable because MicroGEM extraction buffers are compatible with downstream reactions. Primers and PCR reagents can be added directly to the extract with no further purification.

 

Insect

Insects eat between 5-20% of the most important grain crops produced around the world. Further, insect-related vector-borne diseases account for more than 17% of all infectious diseases, causing more than 700,000 deaths annually and millions of cases of dengue, malaria, trypanosomiasis, Lyme disease and many more. Much of this destruction and disease is preventable with timely interventions and prevention strategies.

There are many reasons why it is important to identify an insect at the species level such as guiding insecticide control measures, identifying quarantine pests, and distinguishing disease vector insects. These types of assessments need to be conducted with high levels of accuracy while maintaining high rates of throughput.

The DNA extraction process is often complicated when the sample material is very small and difficult to manipulate such as the legs of insects, larvae and/or eggs. Historically, extracting DNA from a small sample may result in tremendous variations in yield and quality.

MicroGEM's prepGEM Universal reagent kit provides rapid and simple single-tube extractions of single-stranded DNA from intact or crushed insects and insect parts, either fresh or frozen. The single-tube method enables extraction from small samples, even one insect, and ensures all of the nucleic acid in the sample is preserved.

 

Mouse or Rat Tails 

Biomedical research frequently uses rodents as animal models. In many cases, precise genotypes of the animals are necessary, but often this step is time-consuming.

With the number of animals required to generate data, reagent and labor costs can become prohibitive. This problem can be solved with efficient, low-cost DNA extraction.

PDQeX prepGEM Universal is an automated solution for producing quality DNAs in a low-cost automated system. The DNA is ideal for PCR and qPCR and, with qPCR/HRM, can be used for sexing the animal far more reliably than judging the anogenial distance.

 

Blood – Life Sciences

prepGEM Universal leverages our thermophilic proteinase which is activated with a simple temperature change. The whole process is easily automated in a single tube using standard laboratory equipment. A standard thermocycler can be programmed to carry out the extraction.

Comparison of MIcroGEM’s temperature-controlled, closed tube, simplified extraction to conventional approaches that use multiple steps, detergents, and denaturants

 

Example Results: Liquid Blood Extractions

5 µl of whole blood from an EDTA BD Vacutainer® and fresh blood from a finger-prick was extracted using the PDQeX prepGEM kit. 5 µl of the extract (total volume 100 µl) was used in a 20 µl qPCR reaction. The plots of the two samples are shown below.


BRCA 1, qPCR plots showing fresh blood (Black) and EDTA (Blue) with human male standard DNA (Red) at concentrations of: 10 ng/µl, 2 ng/µl, 0.4 ng/µl, 0.08 ng/µl.

Overview

Quick Start Guide

Sheet

Technical Overview

Application Notes:

Fast and Simple DNA preparation for the screening of CRISPR/Cas9-generated Knockout Cell Lines
Rapid extraction of DNA from limited FFPE material derived from lung tumour of engineered mice
Rapid DNA extraction from human saliva and blood for SNP calling

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