NucleoSpin RNA Midi (20)

Macherey-NagelSKU: MN-740962.20



Midi spin kit for isolation of RNA of highest integrity



Application Isolation of RNA
Target RNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Midi prep
Package unit 20 Prep(s)
Handling Centrifugation
Lysate clarification Centrifugation, NucleoSpin Filter Midi
Automated use No
Sample material Animal tissue, Bacteria, Cells, Human tissue, RNA solution, Yeast
Sample amount < 5 x 10⁷ cultured cells, < 200 mg human / animal tissue , < 10¹⁰ bacterial cells, < 3 x 10⁸ yeast cells
Fragment size > 200 nt
Typical yield 180 µg (10⁷ HeLa cells), 620 µg (4 x 10⁷ HeLa cells)
Theoretical binding capacity 700 µg
Typical purity A260/A280 1.9–2.1
Typical RIN > 9
Elution volume 500–1000 µL
Preparation time 80 min/4 preps
Typical downstream application Chip hybridization, Northern blotting, qRT−PCR, RNA-Seq
Storage temperature 15−25 °C
Shelf life (from production) 24 Months
Scope of delivery NucleoSpin RNA Midi Columns, NucleoSpin RNA Filters Midi, Elution Tubes, buffers, RNase-free rDNase
Hazardous material Yes




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